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Experiments were designed to investigate the certain chemical factors and biological examination of water with reference to zooplanktons to establish a relationship between the physico-chemical parameters and population density of water of Peddacheruvu Lake, L.B. Nagar, Hyderabad (T.S). These are the findings of the 192 samples of water which were analyzed during the two years study from four representative sampling (S1, S2, S3 and S4) sites of the water body. The maximum amount of calcium recorded was 58 ppm in the month of May 2012 at S4 station and the minimum quantity of calcium recorded was 39 ppm in the month of August 2011 at S1 station. The maximum value of magnesium recorded was 28 ppm in the month of November 2011 at S1 and the minimum value of 20 ppm was recorded in the month of May 2011 at S3.The chemical factors which have been taken for discussion in the present study are calcium and magnesium to understand the hardness of water and its possible relationship with zooplanktons. The range of Tetrahymena pyriformis observed was from 23,000 at S3 in September 2012 to 40,000 at S1 in May 2011. The range of Keratella quadrata observed was from 14,000 at S4 in September 2012 to 25,000 at S1 in April 2011.The preliminary studies of the water body has confirmed a clear relationship between hardness and population density of Keratella. The highest number of Keratella is directly proportional to quantities of calcium and magnesium.