THE MEDICINAL EFFECT OF BELLADONNA (Atropa belladonna) EXTRACT FOR ABORTION IN THE INDIAN RAT (Rattus rattus)

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Published: 2021-11-29

Page: 426-431


PRIYADARSHI SHIREESH *

Department of Zoology, Dr. Ghanshyam Singh P. G. College, Soyepur, Varanasi, India.

MISHRA DEVBRAT

Department of Zoology, T. D. College, Jaunpur, India.

*Author to whom correspondence should be addressed.


Abstract

Belladonna is important herbal medicine popular in India. About 100 pregnant female Indian rats were experimented by administrating the extract of Belladonna to study the effect for abortion. Rats showed the different behavior when the different doses were administered in their body by injection. The first cage was used as control while the 2nd 3rd 4th 5th cages was used as experimental animals inhabited 10 pregnant female in each cage. The control cage were not supplied  any dose while cage 2nd were supplied 20ppm of Belladonna extract resulted abnormal behavior in all the test animals but the not any abortion or death have been reported. The 3rd cage was supplied 40ppm of belladonna extract showing abnormal behavior in 80% cases while abortion in 20% cases but no case of death of test animals have been reported. In the 4th cage 60ppm belladonna extract were supplied showed abnormality in 60% animals, abortion in 40% animals was found and no death is reported. In the 5th cage 80ppm belladonna abstract was supplied resulted abnormal behavior in 40% animals, abortion in 60% cases and death in 10% test animals. Cage 6th showed not the abnormal behavior in any test animal but abortion in 100% cases followed by death in 80% animals with abortion. The correlation test have been conducted between the concentration of extract and rate of abortion have been estimated as +0.758256 showing a high  degree of positive correlation as the concentration of extract is increasing the abortion rate also increases. The correlation test also conducted between extract concentration and death rate showed very low positive correlation i.e. +0.123476. The F- Test (Anova) have been conducted to check the significance of data, the F- value have been calculated as 3.92 at 5 degree of freedom and 10% probability less than the tabulated value 4.74 therefore data is significant.

Keywords: Ppm, extract, pregnancy termination, abnormality, correlation


How to Cite

SHIREESH, P., & DEVBRAT, M. (2021). THE MEDICINAL EFFECT OF BELLADONNA (Atropa belladonna) EXTRACT FOR ABORTION IN THE INDIAN RAT (Rattus rattus). Asian Journal of Advances in Medical Science, 3(1), 426–431. Retrieved from https://www.mbimph.com/index.php/AJOAIMS/article/view/2639

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References

Biswas TK, Mukherjee B. Plant medicines of Indian origin for wound healing activity: a review. The international Journal of Lower Extremity Wounds. 2003; 2(1):25-39.

Jimenez PA, Rampy MA. Keratinocyte growth factor-2, accelerates wound healing in incisional wounds. Journal of Surgical Research. 1999;81(2):238-42.

Higgs GA, Moncada S, Vane JR. Eicosanoids in inflammation. Ann. Clin. Res. 1984;16:287- 299.

Di Rosa M, Willoughby DA. Screens for anti-inflammatory drugs. J Pharm. Pharmacol. 1971;23:297-303.

Salvemini D, Wang ZQ, Bourdon DM, Stern MK, Currie MG, Manning PT.Evidenceof peroxynitrite involvement in the Carrageenan induced rat paw edema Eur. J. Pharmacol. 1996;303:217-224.

Cowan MM. Plants products antimicrobial agents .Clin. Microbial. Rev.1999;14:564- 584.

Toporcer T, Grendel T, Vidinský B, Gál P, Sabo J, Hudák R. Mechanical properties of skin wounds after Atropa belladonna application in rats. Journal of Metals, Materials and Minerals. 2006;16(1):25-9.

Winter CA, Risley EA, Nuss GW. Carrageenin induced oedema in the hind paw of the rat as an assay for anti-inflammatory drug. Proc. Soc. Exptl. Biol. Med. 1962;111:544-547.

Hernandez PM, Rabanal Gallego R. Evaluation of the anti- inflammatory and analgesic activity of Sideritis anariensis var. pannosa in mice. J. Ethnopharmacol. 2002;81:43-47.

Lowry OH, Rosebrough NJ, Far AL, Randall RJ. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951;193: 265-275.

Gillitzer R, Goebeler M. Chemokines in cutaneous wound healing. Journal of Leukocyte Biology. 2001;69(4):513-21.

Stadler I, Lanzafame RJ, Evans R, Narayan V, Dailey B, Buehner N, et al. 830‐nm irradiation increases the wound tensile strength in a diabetic murine model*. Lasers in Surgery and Medicine. 2001;28(3):220-6.

Midwood KS, Williams LV, Schwarzbauer JE. Tissue repair and the dynamics of the extracellular matrix. The International Journal of Biochemistry and Cell Biology. 2004;36(6):1031-7. 15.

Cytokines in Dermal Regenerative Medicine (Part 1 of a Series); 2012. [Cited 2015 18/4/2015]. Available:http://barefacedtruth.com/2012/06/29/cytokines-in-dermal-regenerative-medicine-part-1/

Enoch S, Price P. Cellular, molecular and biochemical differences in the pathophysiology of healing between acute wounds, chronic wounds and wounds in the aged. World Wide Wounds. 2004;1-16.

Gál P, Vasilenko T, Kováč I, Kostelníková M, Jakubčo J, Szabo P, et al. Atropa belladonna L. water extract: modulator of extracellular matrix formation in vitro and in vivo. Physiological Research. 2012;61(3):241-50.

Mitchell RN, Cotran RS. In: Robinsons Basic Pathology, editn 7.Harcourt Pvt. Ltd., New Delhi, India.2000;33-42.

Ialenti A, Ianaro A, Moncada S, Di Rosa M. Modulation of acute inflammation by endogenous nitric oxide. Eur. J. Pharmacol. 1995;211:177-184.

Kumarasamyraja D, Jeganathan N, Manavalan R. A review on medicinal plants with potential wound healing activity. International Journal of Pharmaceutical Sciences. 2012;2:105-11.

Ahmad F, Khan RA, Rasheed S. Study of analgesic and anti inflammatory activity from plant extracts of Lactuca scariola and Artemisia absinthium. J. Isl. Acad. Sci. 1992;5:111-114.