EFFICACY OF ANTI-BACTERIAL AND ANTI-FUNGAL ACTION ON THREE MEDICINAL PLANTS EXTRACT THE Rosa gallica, Psidium guajava AND Vitis vinifera AGAINST Streptococcus mutans AND Candida albicans -AN In-vitro STUDY
Asian Journal of Advances in Medical Science, Volume 4, Issue 4,
Page 12-18
Abstract
Introduction: World health organization has been an initiative for the widespread practice of herbal medicine been practiced in developing countries and created a scientific basis to treat infection.
Aim: The aim of the study was to test the anti-bacterial and anti-fungal activity of three medical plant extract the Rosa gallica, Psidium guajava L and Vitis vinifera against the streptococcus Mutans and Candida albicans organisms.
Settings and Design: An in-vitro study was conducted collaborated with the department of microbiology and the Department of Public Health Dentistry in SRM Dental College and Hospital for a period of four months. The extract of medical plant of Rosa gallica, Psidium guajava L and Vitis vinifera was tested in different concentration to find out the effective anti-bacterial and anti-fungal action.
Methods and Materials: Extract obtained from the medicinal plant of Rosa gallica, psidium guajava L and Vitis vinifera was procured and powdered. The minimum inhibitory concentration of the obtained extracts was determined by using the tube dilution method and the bacterial strains of Streptococcus mutans and Candida albicans was cultured into the extract obtained at concentration of 1 mg/ml, 500 µg/ml, 250 µg/ml, and 100 µg/ml respectively.
Statistical Analysis Used: Descriptive statistics was done to analysis the bacterial count in different concentration.
Results: The extracts obtained from Rosa gallica and psidium guajava L had a better anti-bacterial property when compared to the other medicinal plants and the extracts obtained from Rosa gallica and psidium guajava L had a better anti-fungal property when compared to the other two medicinal plants.
Conclusions: These plants extracts showed anti-bacterial property in previous studies to add on to the review of literature in our study it proved that Rosa gallica and psidium guajava L had a better anti-fungal activity when compared to another two medicinal plant extract. The clinical significance of the medicinal plant extract can be effectively used as anti-bacterial and anti-fungal agent as it was compared with the gold standard values of kanamycin and Ketoconazole.
Keywords:
- Anti-bacterial
- anti-fungal
- minimum inhibitory concentration and medicinal plant
How to Cite
References
Barani K, Manipal S, Prabu D, Ahmed A, Adusumilli P, Jeevika C. Anti-fungal activity of Morindacitrifolia (noni) extracts against Candida albicans: An in vitro study. Indian Journal of Dental Research. 2014;25(2):188.
Boskabady MH, Shafei MN, Saberi Z, Amini S. Pharmacological effects of Rosa damascena. Iranian journal of basic medical sciences. 2011 Jul;14(4):295.
Dilhuydy JM. Patients′ attraction to complementary and alternative medicine (CAM): A reality which physicians can neither ignore nor deny. Bull Cancer 2003;90:623-8
Díaz-de-Cerio E, Verardo V, Gómez-Caravaca A, Fernández-Gutiérrez A, Segura-Carretero A. Health effects of Psidium guajava L. Leaves: An overview of the last decade. International journal of molecular sciences. 2017;18(4):897.
El Astal ZY, Ashour AE, Kerrit AA. Antimicrobial activity of some medicinal plant extracts in Palestine. Pak. J. Med. Sci. 2005;21(2):187-93.
Kumarasamy B, Manipal S, Prabu Duraisamy AA, Mohanaganesh SP, Jeevika C, Role of aqueous extract of Morindacitrifolia (Indian noni) ripe fruits in inhibiting dental caries-causing Streptococcus mutans and Streptococcus mitis. Journal of Dentistry (Tehran, Iran). 2014;11(6):703
Lee MH, Nam TG, Lee I, Shin EJ, Han AR, Lee P, Lee SY, Lim TG. Skin anti‐inflammatory activity of rose petal extract (Rosa gallica) through reduction of MAPK signaling pathway. Food Science & Nutrition. 2018 Nov;6(8):2560-7.
Ochir S, Nishizawa M, Park BJ, Ishii K, Kanazawa T, Funaki M, Yamagishi T. Inhibitory effects of Rosa gallica on the digestive enzymes. Journal of natural medicines. 2010 Jul 1;64(3):275-80.
Rojas JJ, Ochoa VJ, Ocampo SA, Munoz JF. Screening for antimicrobial activity of ten medicinal plants used in Colombian folkloric medicine: A possible alternative in the treatment of non-nosocomial infections. BMC Complement Altern Med 2006;6:2
Rekha, V., Jayamathi, R., Vijayalakshmi, D., Prabu, N.K. and Sunayana Manipal, K., 2014. Anti cariogenic effect of Terminalia chebula. Journal of clinical and diagnostic research: JCDR, 8(8), p.ZC51.
Sarangowa O, Kanazawa T, Nishizawa M, Myoda T, Bai C, Yamagishi T. Flavonol glycosides in the petal of Rosa species as chemotaxonomic markers. Phytochemistry. 2014 Nov 1;107:61-8.
Swetha R, Shobana R, Sunayana M, Prabu D, Raj Mohan and Naveen Raj. Antibacterial and Antifungal Efficacy of Rose Petal (Rosa Indica) Extract on Oral Microbes - An In Vitro Study. Research & Reviews: Journal of Dental Sciences. 2019;7(2).
Shireen F, Manipal S, Prabu D. Anti-fungal activity of Aloe vera: In vitro study. SRM Journal of Research in Dental Sciences. 2015;6(2):92.
Van der Watt E, Pretorius JC. Purification and identification of active antibacterial component in Carpobrotus edulis L. J Ethnopharmacol. 2001;76:87-91
Vijaya K, Ananthan S. Microbiological screening of Indian medicinal plants with special reference to enteropathogens. J Altern Complement Med. 1997;3:13-20.
-
Abstract View: 145 times
PDF Download: 79 times
