Effects of Selenium and Zinc in Aflatoxin B1 Poisoning in Broiler
Asian Journal of Advances in Medical Science, Volume 5, Issue 1,
Page 65-74
Abstract
To test the effect of selenium & zinc in aflatoxin B1 poisoning in broiler, day-old broiler chicks (n=80) were divided into 5 treatment groups with 16 birds each, (T0-control; T1-50ppb AFB1 T2-100ppb AFB1, T3-200ppb AFB1, T4-400ppb AFB1), after treating 3 weeks, 8 birds were sacrificed & rest of the birds continued with AFB1 & started a new treatment with Zn & Se supplements at the dose rate Zn (40, 60, 80 mg/kgbwt) and Se (30, 40, 50 µg/kgbwt) respectively in T2, T3 & T4 from 3rd week to 5th week. Experiment was conducted from day 1 to 35 days of age. The broilers were weighed, bled, killed by cervical dislocation .Body weights were significantly decreased (643.66±3.17) by 400ppb aflatoxin B1 (P < 0.05) at 21 days compare to control group T0. Higher concentration aflatoxin B1 (400ppb) in T4 group significantly decreased body weight from T1 (50ppb). Aflatoxin B1 induced a significant increase biochemical parameters such as serum alanine transaminase (ALT) & aspartate transaminase (AST).Significantly elevated level (P < 0.05) of ALT & AST were observed in T4 (58.2±0.7 & 1185±17) respectively. In case of hematological parameters, the study revealed that it also reduced the level of total erythrocyte count (TLC), total leucocyte count (TLC), hemoglobin, lymphocyte, monocyte & basophil, however it accelerated neutrophil & eosinophil level. Aflatoxin B1 raised weight of liver, spleen & diminished weight of pancreas. Higher concentration extended mortality rate upto 15% in T4 group at 3 weeks. After treating with Zn& Se, growth performances had been improved gradually, it depressed these negative effects of AFB1 in broiler. These effects of AFB1 were ameliorated by supplementation of Zn & Se. It was concluded that aflatoxin contamination (50, 100, 200, 400 ppb) in broiler diet impaired the performance in terms of body weight gain, biochemical, hematological parameter, relative organ weights & livability percentage. Supplementation of zinc & Se in the aflatoxin contaminated diet improved the adverse effects of aflatoxicosis on performance of the broiler chickens. Supplementation. In conclusion, the results demonstrated that mineral treatment (Zn & Se) might be used for correction of aflatoxin B1 in broiler.
- Aflatoxin B1
- aflatoxicosis
- alanine transaminase alanine aminotransferase
- lymphocytopenia
- monocytopenia total erythrocyte count total leucocyte count
- parts per billion
How to Cite
References
Murugesan GR, Ledoux DR, Naehrer K, Berthiller F, Applegate TJ, Grenier B, Phillips TD, Schatzmayr G. Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poult Sci. 2015;94(6):1298-315.
Lizárraga-Paulín, Eva G. Ernesto Moreno-Martínez, and Susana P. Miranda-Castro. Aflatoxins and their impact on human and animal health: an emerging problem. Aflatoxins-Biochemistry and molecular biology. 2011;13:255-262.
Abdel-Wahhab MA, Nada SA, Khalil FA. Physiological and toxicological responses in rats fed aflatoxincontaminated diet with or without sorbent materials, Animal Feed Science and Technology. 2002;97:3-4:209–219,
Eraslan G, Liman BC, G¨uc¸l¨u BK, Atasever Koc AN, Beyaz L. Evaluation of aflatoxin toxicity in Japanes quails given various doses of hidrated sodium calcium aluminosilicate, Bulletin of the Veterinary Institute in Pulawy. 2004;48:511–517.
Royes JB, Yanong RP. Molds in Fish Feeds and Aflatoxicosis, University of Florida, Institute of Agricultural Sciences (UF/ IFAS); 2002.
Elik IC, Oguz H, Demet O¨et al. Embryotoxicity assay of aflatoxin produced by Aspergillus parasiticus NRRL 2999,British Poultry Science. 2000;41(4): 401–409.
Raju MVLN, Devegowda G. Influence of esterified glucomannanon performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combinedmycotoxicosis (aflatoxin, ochratoxin and T-2 toxin), British Poultry Science. 41(5):640–650.
Basmacioglu H, Oguz H, Ergul M, ¨Ol RC¸, Birdane YO. Effect of dietary esterified glucomannan on performance, serum biochemistry and haematology in broilers exposed to aflatoxin, Czech Journal of Animal Science. 2005;50(1):31–39.
D¨onmez N, Keskin E. The effects of aflatoxin and glucomannan on coagulation parameters in rabbits, Veterinarski Arhiv. 2009;79(6):555–560.
Hamilton PB, Tung HT, Harris JR, Gaines JH, Donaldson WE. The effect of dietary fat on aflatoxicosis in turkeys. Poultry Science. 1972;51:165–70.
Smith JW, Hill CJ, Hamilton PB. The effect of dietary modifications on aflatoxicosis in broiler chicken. Poultry Science. 1971;50:768–74.
Lanza GM, Washburn KW, Wyatt RD. The effect of linoleic acid on broiler response to graded levels of aflatoxin. Arch. Geflugelk. 1981;45:206–11.
Hegazy SM, Adachi Y. Comparison of the effects of dietary selenium, zinc, and selenium and zinc supplementation on growth and immune response between chick groups that were inoculated with Salmonella and aflatoxin or Salmonella. Poultry Science. 2000;79: 331–35.
Yilmaz B, Fizyoloji, Feryal Matbaacılık, Ankara. Turkey, 2nd edition; 2000.
SPSS 16.0., SPSS Inc.; 2007.
Dersjant-Li Y, Verstegen MWA, Gerrits WJJ. The impact of low concentrations of aflatoxin, deoxynivalenol or fumonisin in diets on growing pigs and poultry. Nutr. Res. 2003;16:223–239.
Raju MVLN, Devegowda G. Influence of esterified-glucomannan on performance and organ morphology, serum biochemistry and haematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin) Br. Poult. Sci. 2000;41: 640–650.
DOI: 10.1080/713654986.
Tedesco D, Steidler S, Galletti S, Tameni M, Sonzogni O, Ravarotto L. Efficacy of silymarin-phospholipid complex in reducing the toxicity of aflatoxin B1 in broiler chicks. Poult. Sci. 2004;83:1839–1843.
Hegazy SM, Adachi Y. Comparison of the effects ofdietary selenium, zinc, and selenium and zinc supplementationon growth and immune response between chick groups thatwere inoculated with Salmonella and aflatoxin or Salmonella.Poultry Science. 2000;79: 331–35.
Mamta Sharma, Ram Singh, Mandal and Vivek, Prasad Gupta. Efficacy of zinc in amelioration of aflatoxicosis in broiler chickens. Indian Journal of Animal Sciences. 2014;84(3):311–315.
Tessari, Eliana NC, Estela Kobashigawa, Ana Lucia SP, Cardoso, Ledoux, David R, Rottinghaus, George E, Oliveira, Carlos AF. Effects of AflatoxinB1 B1 and Fumonisin B1 on Blood Biochemical Parameters in Broilers. Toxins. 2010;2:453-460.
Agag BI. Mycotoxins in foods and feeds: Aflatoxins. Association of Universal Bullettin of Environmental Research. 2004;7(1):173-191.
Prameela Rani M, Nissar Ahmad N, Eswara Prasad P, Sri Latha Ch. Hematological and Biochemical changes of stunting syndrome in Broiler chicken. Veterinary World. 2011;4(3):124-125.
Shameem Ara Begum, Tirtha Nath Upadhyaya, Taibur Rahman, Debesh Chandra Pathak, Kavita Sarma, Chandana Choudhury Barua, RS Bora.. Hematobiochemical and pathological alterations due to chronic chlorpyrifos intoxication in indigenous chicken. Indian journal of Pharmacology. 2015;47(2): 206-211.
Yohannes T, Sharma AK, Singh SD, Sumi V. Experimental haematobiochemical alterations in broiler chickens fed with t-2 toxin and co-infected with IBV. Open Journal of Veterinary Medicine. 2013; 3:252-258.
Abdel-Wahhab MA, Nada SA, Khalil FA. 2002. Physiological and toxicological responses in rats fed aflatoxin contaminated diet with or without sorbent materials, Animal Feed Science and Technology, vol. 97, no. 3-4, pp. 209–219.
Basmacioglu H, Oguz H, Ergul M, ¨ol RC, Birdane YO. Effect of dietary esterified glucomannan on performance,serum biochemistry and haematology in broilers exposed to aflatoxin, Czech Journal of Animal Science. 2005;50(1):31–39.
Ogˇuz H, Kec T ¸eci, Birdane YO, Nder FO¨, Kurtogˇlu V. Effect of clinoptilolite on serum biochemical and haematological characters of broiler chickens during aflatoxicosis, Research in Veterinary Science. 2000;69(1):89–93.
Elik IC¸, Oguz H, Demet O¨, Do¨nmez HH, Boydak M, Sur E. Efficacy of polyvinylpolypyrrolidone in reducing the immunotoxicity of aflatoxin in growing broilers, British Poultry Science. 2000; 41(4):430–439.
Elik IC¸, Oguz H, Demet O¨ et al. Embryotoxicity assay of aflatoxin produced by Aspergillus parasiticus NRRL 2999,”British Poultry Science. 2000; 41(4):401–409.
Kec¸eci T, Oguz H, Kurtoglu V, Demet O¨. Effects of polyvinylpolyprolidone, synthetic zeolite and bentonite on serum biochemical and haematological characters of broiler. The Scientific World Journal chickens during aflatoxicosis,” British Poultry Science. 1998;39:452–458.
Raju MVLN, Devegowda G. Influence of esterified glucomannan on performance and organ morphology, serum biochemistry and hematology in broilers exposed to individual and combined mycotoxicosis (aflatoxin, ochratoxin and T-2 toxin). British Poultry Science. 2000;41: 640–50.
Kubena LF, Harvey RB, Phillips TD, Huff WE. Modulation of aflatoxicosis in growing chickens by dietary addition of a hydrated sodium calcium aluminosilicate. Poultry Science. 1998;67: 106.
Silambarsan S. ‘Efficacy of diatomaceous earth, sodium bentonite and zeolite as aflatoxin adsorbant in broiler chickens.’ M.V.Sc. Thesis submitted to Deemed University, IVRI, Izatnagar; 2011.
Abaji PK. ‘Efficacy of Saccharomyces cerevisiae and mannan oligosaccharides (MOS) in counteracting aflatoxicosis in broiler chickens.’ M.V. Sc. Thesis, submitted to Deemed University, IVRI, Izatnagar; 2012.
Denli M, Blandon JC, Guynot ME, Salado S, Perez JF. Effects of dietary Afladetox on performance, serumbiochemistry, histopathological changes and aflatoxin residues in broilers exposed to aflatoxin B1. Poultry Science. 2009;88: 1444–51.
Gopi K. ‘Influence of melatonin on aflatoxicosis in broiler chickens.’ M.V.Sc. Thesis submitted to Deemed University, IVRI, Izatnagar; 2006.
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