PHYTOCHEMICAL SCREENING AND BIOACTIVITY OF Zingiber officinale TO COMBAT THE MULTIDRUG-RESISTANT BACTERIAL PATHOGENS USING FOLDSCOPE

Main Article Content

K. ABIRAMI
M. MAGHIMAA

Abstract

Background: Foldscope, is a paper-based, portable microscope with a magnification power of 140X. Zingiber officinale is widely used as spice, flavor and therapeutic plant in folk and conventional medicines. It is used in the regular diet in many asian countries. Chemical analysis of ginger shows that it consists of more than 400 different compounds.

Aim: In the present study, to isolate and identify the skin infection causing multidrug-resistant bacterial pathogens from clinical specimens using Foldscope (a paper microscope). To evaluate the phytochemicals and antimicrobial activity of aqueous extract of Zingiber officinale rhizome against the isolated MDR bacterial pathogens.

Materials and Methods: The isolates were examined by Foldscope microscopy and biochemical tests. Phyto-chemical study analyzed the existence of alkaloids, flavonoids, phenols, saponins, terpenoids, steroids, tannins, reducing sugars, carbohydrates, and amino acids. Chloramphenicol, Penicillin, Streptomycin, Methicillin, and Vancomycin, are the antibiotic disc used in the disc diffusion assay.

Results: The pathogens were isolated using Foldscope. Phytochemical study revealed the presence of flavonoids, tannins, carbohydrates and reducing sugars, saponins, Glycosides, terpenoids, and steroids. Antimicrobial activity was performed by Kirby-Bauer’s–agar well diffusion method using aqueous extract of Z. officinale against the pathogens from wound and multidrug-resistant pattern was observed in the isolates. The crude aqueous extract of Z. officinale rhizome was found to be active against multidrug-resistant Staphylococcus aureus (20 mm), Escherichia coli (19 mm), Pseudomonas aeruginosa (22 mm) and Klebsiella pneumonia (19 mm). The highest zone of clearance was found in Pseudomonas aeruginosa (22 mm), followed by Staphylococcus aureus (20 mm), Escherichia coli (19 mm), and Klebsiella pneumoniae (19 mm).

Conclusion: The end result attained that the aqueous extracts have a potential resource of useful pharmaceutical bioactive and antibacterial property and it is used as a natural drug by means of itself to treat the microbial infection.

Keywords:
Phytochemicals, antimicrobial, wound pathogens, multidrug-resistance.

Article Details

How to Cite
ABIRAMI, K., & MAGHIMAA, M. (2019). PHYTOCHEMICAL SCREENING AND BIOACTIVITY OF Zingiber officinale TO COMBAT THE MULTIDRUG-RESISTANT BACTERIAL PATHOGENS USING FOLDSCOPE. UTTAR PRADESH JOURNAL OF ZOOLOGY, 40(2), 67-74. Retrieved from https://www.mbimph.com/index.php/UPJOZ/article/view/1428
Section
Original Research Article

References

Diwan J, Chikkanaragund K, Suma TC, Mahadevaswamy YS, Amaresh PR, Badariprasad, Lokesh R. Assessment of agro biodiversity through the foldscope. Plant Science and Natural Medicines. Allied Academics. Journal of Agricultural Science and Botany. 2018;2:14.
DOI: 10.4066/2591-7897-C1-002

Cybulski JS, Clements J, Prakash M. Foldscope: Origami-based paper microscope. PLOS ONE. 2014;9:e98781.

Sharma AD. Foldscopebased methods to detect in-tissue antioxidant activity and secondary metabolites in pollen and stomata of Lantana camara. Research & Reviews in Biotechnology & Biosciences. 2018;5(1):29-33.
DOI: 10.6084/m9.figshare.8977313.v1

Parekh J, Chanda V. In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants. Turkish J Biol. 2007;31:53–8.

Rahman HMA, Ahmed K, Rasool MF, Imran I. Pharmacological evaluation of smooth muscle relaxant and cardiac-modulation potential of Phyla nodiflora in ex-vivo and in-vivo experiments. Asian Pacific Journal of Tropical Medicine. 2017;10(12):1146–1153.

Hasan I, Hussain MS, Millat MS, et al. Ascertainment of pharmacological activities of Allamanda neriifolia Hook and Aegialitis rotundifolia Roxb used in Bangladesh: An in vitro study. Journal of Traditional and Complementary Medicine. 2018;8(1):107-112.

Christenhusz MJM, Byng JW. The number of known plants species in the world and its annual increase. Phytotaxa. 2016;261(3):201-217.

Phuaklee P, Sakpakdeejaroen I, Itharat A. Cytotoxic and antioxidant activities of two species of ginger extracts. Thai Journal of Pharmacology. 2010;32(1):82-85.

Xu Z, Chang L. Zingiberaceae. In: Xu Z, Chang L, Eds. Identification and Control of Common Weeds: Singapore: Springer Link. 2017;3:909-911.

Yang Z, Yang W, Peng Q, He Q, Feng Y, Luo S, Yu Z. Volatile phytochemical composition of rhizome of ginger after extraction by headspace solid-phase microextraction, petroleum ether extraction and steam distillation extraction. Bangladesh J Pharmacol. 2009;4:136-143.

Sivasothy Y, Chong WK, Abdul Hamid, Eldeen IM, Sulaiman SF, Awang K. Essential oils of Zingiber officinale var. rubrum Theilade and their antibacterial activities. Food Chem. 2011;124:514-517.

Abdel-Azeem AS, Hegazy AM, Ibrahim KS, Farrag AR, El-Sayed EM. Hepatoprotective, antioxidant, and ameliorative effects of ginger (Zingiber officinale Roscoe) and vitamin E in acetaminophen treated rats. J Diet Suppl. 2013;10:195-209.

Jeena K, Liju VB, Kuttan R. Antioxidant, anti-inflammatory and antinociceptive activities of essential oil from ginger. Indian J Physiol Pharmacol. 2013;57:51-62.

Mostafa NM, Singab AN. After HCV eradication with Sovaldi®, can herbs regenerate damaged liver, minimize side effects and reduce the bill? Med Aromat Plants. 2016;5:257.

Ramsi V, Nivedhitha K, Abirami K, Maghimaa M. Phytochemical screening and antibacterial properties of Punica granatum extracts against gastrointestinal infection an in-vitro study. Uttar Pradesh Journal of Zoology. 2019;40(1): 25-32.

Herborne JB. Phytochemical methods 3rd Edn. London: Chapman and Hall Ltd. 1973;135-203.

Okwu DE. Evaluation of the chemical composition of indigenous species and flavoring agents. Global J. Pure Appl Sci. 2001;7(3):455–9.

Rahilla TN, Rukh S, Ziaidi AA. Phytochemical screening of medicinal plants belonging to Euphorbiaceae. Pak Vet J. 1994;14:160–2.

Sofowara A. Medicinal plants and traditional medicine in Africa. Spectrum Book LTD, Ibadan, Nigeria. 1993;289.

Putta S, Kilari EK. Protective activity of aqueous pericarp extract of Punica granatum against hyperglycemia-induced by streptozotocin in rats. Biosci., Biotechnol. Res. Asia. 2014;11:1439−1446.

Evans M, Wilson D, Guthrie NA. Randomized, double-blind, placebo-controlled, pilot study to evaluate the effect of whole grape extract on antioxidant status and lipid profile. J. Funct. Foods. 2014;7:680−691.

Liu W, Ma H, Frost L, Yuan T, Dain JA, Seeram NP. Pomegranate phenolics inhibit formation of advanced glycation end products by scavenging reactive carbonyl species. Food Funct. 2014;5:2996−3004.

Da Silva JK, Cazarin CBB, Correa LC, Batista ÂG, Furlan CPB, Biasoto ACT, Pereira GE, de Camargo AC, Marostica Junior MR. Bioactive compounds of juices from two Brazilian grape cultivars. Journal of the Science of Food and Agriculture; 2015.
DOI: 10.1002/jsfa.7309

Sun S, Kadouh HC, Zhu W, Zhou K. Bioactivity-guided isolation and purification of α-glucosidase inhibitor, 6-O -glycosides, from Tinta Cso grape pomace. Journal of Functional Foods. 2016;23:573-579.

Hung WL, Sun Hwang L, Shahidi F, Pan MH, Wang Y, Ho CT. Endogenous formation of trans fatty acids: Health implications and potential dietary intervention. Journal of Functional Foods. 2016;25:14-24.

Kasiwut J, Youravong W, Adulyatham P, Sirinupong N. Angiotensin I-converting enzyme inhibitory and Ca-binding activities of peptides prepared from tuna cooking juice and spleen proteases. Int. J. Food Sci. Tech. 2015;50:389–395.

Wu Q, Jia J, Yan H, Du J, Gui, Z. A novel angiotensin-I converting enzyme (ACE) inhibitory peptide from gastrointestinal protease hydrolysate of silkworm pupa (Bomby.x Mori) protein: Biochemical characterization and molecular docking study. Peptides. 2015;68:17–24.

Ahtesh FB, Stojanovska L, Mathai ML, Apostolopoulos V, Mishra VK. Proteolytic and angiotensin-converting enzyme-inhibitory activities of selected probiotic bacteria. Int. J. Food Sci. Tech. 2016;51:865–874.

Mosele JI, Macia A, Romero MP, Motilva MJ, Rubio L. Application of in vitro gastrointestinal digestion and colonic fermentation models to pomegranate products (juice, pulp and peel extract) to study the stability and catabolism of phenolic compounds. J. Funct. Foods. 2015;14: 529−540.

Ambigaipalan P, Al-Khalifa AS, Shahidi F. Antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities of date seed protein hydrolysates prepared using Alcalase, Flavourzyme and Thermolysin. Journal of Functional Foods. 2015;18:1125-1137.

Ambigaipalan P, Shahidi F. Date seed flour and hydrolysates affect physicochemical properties of muffin. Food Biosci. 2015;12:54−60.

Singh B, Bhat TK. Potential therapeutic applications of some antinutritional plant secondary metabolites. Journ. Agric. Food Chem. 2003;51:5579-5597.

Imokawa G. Recent advances in characterizing biological mechanisms underlying UV-induced wrinkles: A pivotal role of fibrobrast - derived elastase. Arch Dermatol Res. 2008;300:S7-S20.

Thompson EH, Wolf ID, Allen CE. Ginger rhizome: A new source of proteolytic enzyme. J Food Sci. 1973;38:652- 5.

Jagetia GC, Baliga MS, Venkatesh P, Ulloor JN. Influence of ginger rhizome (Zingiber officinaleRosc.) on survival, glutathione and lipid peroxidation in mice after whole-body exposure to gamma radiation. Radiat Res. 2003;160:584-92.

Liu L, Shao W, Lin G. Microcalorimetry studies on the antimicrobial actions of volatile oil of dry ginger. J Therm Anal Calorim. 2012;107(2): 831–5.
Available:https://doi.org/10.1007/s10973-011-1589-3