Main Article Content



Free radicals or highly reactive oxygen species are formed by exogenous chemicals or endogenous metabolic processes in the human body. Recently there has been an upsurge of interest in the therapeutic potentials of medicinal plants as antioxidants in reducing free radical scavenging activity. Vermicompost is an organic materials broken down by the interactions between microorganisms and earthworms in a mesophilic process to produce fully stabilized and organic soil amendments with low carbon to nitrogen (C:N) ratios  Vermicompost is rich in NPK micronutrients, and beneficial soil microbes, so it is garnering attention as a greener replacement for chemical fertilizers to maintain and further improve soil quality. In the present study effects of vermicompost supplementation on antioxidant activities of Trigonella foenum-graecum was investigated.  Anecic earthworm Lampito mauritii, Vermicompost, Farmyard manure and seeds of Trigonella foemum-graecum. L was inoculated in to soil at different treatments. Seeds of Trigonella foemum-graecum treated with 0.1% Mercuric chloride for 2 minutes and washed with running water to remove contamination of seed coat, prior to germination studies. The experimental setup was designed and it was classified in to six groups. Trigonella foenum – graecum was plugged out from the different treatment experimental setup at after 15 days. Antioxidant activity was determined by DPPH free radical scavenging assay. DPPH scavenging activity of ethylacetate, ethanol and aqueous extract of Trigonella  foenum-graecum with different treatments was studied.  Out of which ethanol extracts of Trigonella foenum-graecum exhibits maximum level of DPPH free radical scavenging activity and group IV possessed the good activity when compared to other groups due to presence of vermicompost and Lampito maurtii. The extract from Trigonella foenum- graecum treated with vermicompost and Lampito marutii showed better antioxidant activity when compared to other groups. The present study suggests that Trigonella foenum-graecum could be used as natural antioxidants and nutraceuticals to enhance the health benefits.

Trigonella foenum-graecum, Lampito mauritii, vermicompost, farmyard manure, DPPH activity.

Article Details

How to Cite
Original Research Article


Song X, Liu M, Wu D, Griffiths BS, Jiao J, Li H, Hu F. Interaction matters: Synergy between vermicompost and PGPR agents improves soil quality, crop quality and crop yield in the field. Appl. Soil Ecol. 2015;89:25–34.

Lazar M, Mitre V, Tripon FA, Badiu DE. The potential use of vermicompost in orchards. Bull. UASVM Hortic. 2014;71:56–58.

Halliwell B, Gutteridge JMC. Free Radicals in Biology and Medicine, Ed 4. Clarendon Press, Oxford; 2006.

Devasagayam TPA, Tilak JC, Boloor KK, Ketaki Sane S, Saroj S. Ghaskadbi, Lele RD. Review article. Free radicals and antioxidants in human health: Current Status and Future Prospects. JAPI. 2004;52.

Kaur C, Kapoor HC. Antioxidants in fruits and vegetables-the millennium's health. Int J Food Sci Tech. 2001;36:703–25

Nagulendran KR, Velavan S, Mahesh R, Begum VH. In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. E J Chem. 2007;4:440–9.

Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: Fruits. J Agric Food Chem. 2001;49:5315–21

Sudarajan N, Ahamad H, Kumar V. Cytisus scoparius Link-A natural antioxidant. 2006;6: 1-7.

Blois MS. Antioxidant determinations by the use of a stable free radical, Nature. 1958;181: 1199- 1200.

Reena P, Aditi Patel, Sachin Desai, Anju Nagee. Study of secondary metabolites and antioxidants properties of leaves, stem and root among Hibiscus Rosa- sinensis cultivars. Asian Journal of Experimental Biological Sciences. 2012;3(4):719-725.

Harborne AJ. Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media; 1998.

Naik GH, Priyadarsini KI, Satav JG, Banavalikar MM, Sohoni PP, Biyani MK. Comparative antioxidant activity of individual herbal components used in Ayurvedic medicine. Phytochemistry. 2003;63(1):97-104

Reddy ARK, Grace JR. In vitro evaluation of antioxidant activity of methanolic extracts of selected mangrove plants. Med. Aromat. Plants. 2016;5(3):2167-0412.

Vilioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits , vegetables, and grain products: J Agri Food Chem. 1998;46:4113-4117.

Duan XJ, Zhang WW. Evaluation of antioxidant property of extract andfractions obtained from a red alga, Polysiphonia urceolata. Food Chem. 2006;95:35-43.

Meir S, Kanner J. Determination and involvement of aqueous reducing compounds in oxidative defense systems of various sensing leaves. J Agric Food Chem. 1995;43:1813-1815

Demla M, Verma H. In vitro antioxidant activity,total phenolic and total flavonoid content of different extracts of Solanum xanthocarpum Berries. International Journal of Pharmacy and Pharmaceutical Science. 2012;4: 154-157.

Adithya ES, Sasikumar JM, Krishnakumar KA, Lakshmi MS, Christabel H. In vitro antioxidant activity,mineral content and HPLC analysis of Talinum portulacifolium (forssk.) asch. ex schweinf, leaf & stem. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4:423-429.

Hasan SM, Hossain MM, Faruque A, Mazumder MEH, Rana MS, Akter R, Alam MA. Comparison of antioxidant potential of different fractions of Commelina benghalensis Linn. Bang. J. Life. Sci. 2008;20(2):9-16.

Yusof Z, Ramasamy S, Mahmood N, Yaacob J. Vermicompost supplementation improves the stability of bioactive anthocyanin and phenolic compounds in Clinacanthus nutans lindau. Molecules. 2018;23(6):1345.

Ismail SA. The earthworm book. Other India Press; 2009.