INVITRO DRUG SENSITIVITY OF Rhodococcus equi ISOLATED FROM NORMAL MICRO-FLORA OF SKIN AND UPPER RESPIRATORY TRACT OF EQUINES
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Published
May 5, 2020
Page:
24-29
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DAU LAL BOHRA
Department of Zoology, Seth GB Podar College, Nawalgarh, Jhunjhunu, Rajasthan, India.
Abstract
Rhodococcus equi (R. equi) is asignificant pathogen with equines being the most susceptible host. This facultative intracellular pathogen is quite stable in nature and contaminates equine environments. In this paper aimed to antibiogram study of R. equi. Out of 220 samples processed 37 isolates were found to be R. equiin out of 75 Rhodococcus sp. All the 37 isolates of R. equi were subjected to resistotyping with 16 different antibiotics. All isolates of R. equi showed 100 percent sensitivity to Gentamicin followed by ciprofloxacin and streptomycin (sensitivity-97.30%) while higher resistance i.e. 97.30 percent to furazolidone and 59.46 percent to sulphadiazine was observed.
Keywords:
Rhodococcus equi, antibiogram, sensitivity, gentamicin
Article Details
How to Cite
BOHRA, D. L. (2020). INVITRO DRUG SENSITIVITY OF Rhodococcus equi ISOLATED FROM NORMAL MICRO-FLORA OF SKIN AND UPPER RESPIRATORY TRACT OF EQUINES. UTTAR PRADESH JOURNAL OF ZOOLOGY, 41(5), 24-29. Retrieved from https://www.mbimph.com/index.php/UPJOZ/article/view/1509
Section
Original Research Article
References
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Costa MM, Machado SA, Krewer CC, Ilha MRS, Graca DL, Guaraldi ALM, Vargas AC. Pathogenicity of Rhodococcus equi in mice, isolated from environment, human and horse clinical samples. Pesq Vet Bras. 2006;26 (3):167-170.
Liu H, Wang Y, Yan J, Wang C, He H. Appearance of multidrug-resistant virulent Rhodococcus equi Clinical isolates Obtained in China. J. Clin. Microbiol. 2014;52:703.
Mascellino MT, Iona E, Ponzo R, Mastroianni CM, Delia S. Infections due to Rhodococcus equi in 3 HIV infected patients – microbiological findings and antibiotic susceptibility. J Clin Pharmacol Res. 1994;14: 157–163.
Bohra DL, Maherchandani S, Bahura CK, Kashyap SK. A comparative study of aerobic bacteria in upper respiratory tract of horses and donkeys. Veterinary Practitioner. 2008;9(1):50-51.
Quinn P, Carter ME, Markey BK, Carter GR. Corynebacterium species and Rhodococcus equi. In: Clinical veterinary microbiology. Wolfe Publishing, Mosby-Year Book, Europe Ltd. Lyton House, Tavistock Square, London, England. 1994a;137–143.
Hucker GJ, Conn HJ. Methods of Gram staining. N Y State AgricExpStnTech Bull. 1923;93:3-37.
Cowan ST, Steel KJ. In: Cowan and steel's manual for the identification of medical bacteria, 2nd Ed. Cambridge University Press, Cambridge, New York. 1974;90-93.
Quinn P, Carter ME, Markey BK, Carter GR. Bacterial Pathogens: Microscopy, culture and identification In: Clinical veterinary microbiology. Wolfe Publishing, Mosby-Year Book, Europe Ltd. Lyton House, Tavistock square, London, England. 1994b;21-66.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Tech Bull Regist Med Technol. 1966;36(3):49-52.
Balow’s A, Hauser WJ Jr., Herrmann KL, Isenberg HD, Shadomy HJ Pseudomonas and related genera. In: Gilardi GL. (Ed.), Manual of clinical microbiology, 5th Ed. American Society for Microbiology, Washington, D.C. 1992;429-441.
Sellon DC, Walker K, Suyemoto M, Altier C. Nucleic acid amplification for rapid detection of Rhodococcus equi in equine blood and tracheal wash fluids. Am J Vet Res. 1997;58 (11):1232-7.
Silva P, Miyata M, Sato D, Santos AC, Mendes NH, Leite CQ. Rhodococcus equi isolation from sputum of patients with suspected tuberculosis. Mem Inst Oswaldo Cruz. 2010; 105(2):199-202.
McNeil MM, Brown JM. The medically important aerobic actinomycetes: epidemiology and microbiology. Clin Microbiol Rev. 1994; 7(3):357-417.
Sweeney CR, Sweeney RW, Divers TJ. Rhodococcus equi pneumonia in 48 foals: Response to antimicrobial therapy. Vet Microbiol. 1987;14(3):329-36.
Haites RE, Muscatello G, Begg AP, Browning GF. Prevalence of the virulence-associated gene of Rhodococcus equi in isolates from infected foals. J Clin Microbiol. 1997;35(6): 1642-4.
Bohra DL, Bahura CK, Kashyap SK, Maherchandani S. Upper respiratory tract and skin diseases of horses in Indian desert: case study. Adv. Pharmacol. Toxicol. 2013;14(3): 15-20.
Muscatello G, Gerbaud S, Kennedy C, Gilkerson JR, Buckley T, Klay M, Leadon DP, Browning GF. Comparison of concentrations of Rhodococcus equi and virulent R. equi in air of stables and paddocks on horse breeding farms in a temperate climate. Equine Vet J. 2006;38(3):263-5.
Morton A, Begg A, Anderson G, Takai S, Lammler C, Browning G. Epidemiology of Rhodococcus equi Strains on Thoroughbred Horse Farms. Appl Environ Microbiol. 2001;67 (5):2167–2175.
Meijer WG, Prescott JF. Rhodococcus equi. Vet Res. 2004;35:383-396.
Takai S, Fujimori T, Katsuzaki K, Tsubaki S. Ecology of Rhodococcus equi in horses and their environment on horse-breeding farms. Vet Microbiol. 1987;14(3):233-9.
Costa MM, Machado SA, Krewer CC, Ilha MRS, Graca DL, Guaraldi ALM, Vargas AC. Pathogenicity of Rhodococcus equi in mice, isolated from environment, human and horse clinical samples. Pesq Vet Bras. 2006;26 (3):167-170.
Liu H, Wang Y, Yan J, Wang C, He H. Appearance of multidrug-resistant virulent Rhodococcus equi Clinical isolates Obtained in China. J. Clin. Microbiol. 2014;52:703.
Mascellino MT, Iona E, Ponzo R, Mastroianni CM, Delia S. Infections due to Rhodococcus equi in 3 HIV infected patients – microbiological findings and antibiotic susceptibility. J Clin Pharmacol Res. 1994;14: 157–163.
Bohra DL, Maherchandani S, Bahura CK, Kashyap SK. A comparative study of aerobic bacteria in upper respiratory tract of horses and donkeys. Veterinary Practitioner. 2008;9(1):50-51.
Quinn P, Carter ME, Markey BK, Carter GR. Corynebacterium species and Rhodococcus equi. In: Clinical veterinary microbiology. Wolfe Publishing, Mosby-Year Book, Europe Ltd. Lyton House, Tavistock Square, London, England. 1994a;137–143.
Hucker GJ, Conn HJ. Methods of Gram staining. N Y State AgricExpStnTech Bull. 1923;93:3-37.
Cowan ST, Steel KJ. In: Cowan and steel's manual for the identification of medical bacteria, 2nd Ed. Cambridge University Press, Cambridge, New York. 1974;90-93.
Quinn P, Carter ME, Markey BK, Carter GR. Bacterial Pathogens: Microscopy, culture and identification In: Clinical veterinary microbiology. Wolfe Publishing, Mosby-Year Book, Europe Ltd. Lyton House, Tavistock square, London, England. 1994b;21-66.
Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Tech Bull Regist Med Technol. 1966;36(3):49-52.
Balow’s A, Hauser WJ Jr., Herrmann KL, Isenberg HD, Shadomy HJ Pseudomonas and related genera. In: Gilardi GL. (Ed.), Manual of clinical microbiology, 5th Ed. American Society for Microbiology, Washington, D.C. 1992;429-441.
Sellon DC, Walker K, Suyemoto M, Altier C. Nucleic acid amplification for rapid detection of Rhodococcus equi in equine blood and tracheal wash fluids. Am J Vet Res. 1997;58 (11):1232-7.
Silva P, Miyata M, Sato D, Santos AC, Mendes NH, Leite CQ. Rhodococcus equi isolation from sputum of patients with suspected tuberculosis. Mem Inst Oswaldo Cruz. 2010; 105(2):199-202.
McNeil MM, Brown JM. The medically important aerobic actinomycetes: epidemiology and microbiology. Clin Microbiol Rev. 1994; 7(3):357-417.
Sweeney CR, Sweeney RW, Divers TJ. Rhodococcus equi pneumonia in 48 foals: Response to antimicrobial therapy. Vet Microbiol. 1987;14(3):329-36.
Haites RE, Muscatello G, Begg AP, Browning GF. Prevalence of the virulence-associated gene of Rhodococcus equi in isolates from infected foals. J Clin Microbiol. 1997;35(6): 1642-4.
Bohra DL, Bahura CK, Kashyap SK, Maherchandani S. Upper respiratory tract and skin diseases of horses in Indian desert: case study. Adv. Pharmacol. Toxicol. 2013;14(3): 15-20.
Muscatello G, Gerbaud S, Kennedy C, Gilkerson JR, Buckley T, Klay M, Leadon DP, Browning GF. Comparison of concentrations of Rhodococcus equi and virulent R. equi in air of stables and paddocks on horse breeding farms in a temperate climate. Equine Vet J. 2006;38(3):263-5.
Morton A, Begg A, Anderson G, Takai S, Lammler C, Browning G. Epidemiology of Rhodococcus equi Strains on Thoroughbred Horse Farms. Appl Environ Microbiol. 2001;67 (5):2167–2175.
Meijer WG, Prescott JF. Rhodococcus equi. Vet Res. 2004;35:383-396.
Takai S, Fujimori T, Katsuzaki K, Tsubaki S. Ecology of Rhodococcus equi in horses and their environment on horse-breeding farms. Vet Microbiol. 1987;14(3):233-9.