PROTECTIVE ROLE OF PHYTOFABRICATED GOLD NANOPARTICLES AGAINST Aeromonas hydrophila INDUCED TOXICITY IN Oreochromis mossambicus
F. SHINE *
Fisheries Biotechnology and Nano Science Unit, P.G. and Research Centre, University of Kerala, Department of Zoology, Fatima Mata National College (Autonomous), Kollam 691001, Kerala, India.
A. AKHILA THOMAS
Fisheries Biotechnology and Nano Science Unit, P.G. and Research Centre, University of Kerala, Department of Zoology, Fatima Mata National College (Autonomous), Kollam 691001, Kerala, India.
S. T. SHIBU JOSEPH
P.G. Department of Chemistry, Research Centre, University of Kerala, Fatima Mata National College (Autonomous), Kollam 691001, Kerala, India.
DHANYA RAJ
Fisheries Biotechnology and Nano Science Unit, P.G. and Research Centre, University of Kerala, Department of Zoology, Fatima Mata National College (Autonomous), Kollam 691001, Kerala, India.
*Author to whom correspondence should be addressed.
Abstract
In aquaculture, disease outbreak is a major concern. The use of chemotherapeutic agents in controlling infections leads to drug resistance and residue related issues. Recently, nanoparticles has gained much attention as an alternative to antibiotics since they supersede the side effects of antibiotics. Metal based nanoparticles (NPs) are reported to have excellent antimicrobial efficacy. The study aims to evaluate the antibacterial efficacy of gold nanoparticles (AuNPs) synthesized through chemical and green methods against Aeromonas hydrophila infection in Oreochromis mossambicus (Mossambique tilapia). In the present investigation, green synthesis has been carried out by aqueous extract of Emblica officinalis (Amla) fruit and chemical synthesis by sodium borohydride reduction method. The obtained AuNPs were characterized by Ultraviolet-Visible spectroscopy (UV-Vis spectroscopy) and FTIR (Fourier Transform Infrared Spectroscopy) studies. The antibacterial efficacy of synthesized AuNPs was assessed by the activity of metabolic enzymes (AST, ALT, ALP and LDH) in the selected tissues of Oreochromis mossambicus reared on experimental diet post challenge. The antibacterial investigation revealed green synthesized AuNPs exhibited good antimicrobial activity.
Keywords: Green synthesis, chemical synthesis, gold nanoparticles, Emblica officinalis, Aeromonas hydrophila.