IMPACT OF MALATHION (AN ORGANOPHOSPHATE) ON BIOCHEMICAL CONSTITUENTS (PROTEINS, CARBOHYDRATES AND FREE AMINOACIDS) OF FRESH WATER FISH Channa punctatus (Bloch)
M. VENKATESHWAR RAO
Department of Zoology, Kakatiya University, Warangal, 506009, India.
VENKAIAH YANAMALA
Department of Zoology, Kakatiya University, Warangal, 506009, India.
*Author to whom correspondence should be addressed.
Abstract
Pesticides are most hazardous chemicals causing high risk to the environment, fisheries and people. Organophosphate pesticides are increasingly used in recent years due to their biodegradability and short resistance time in the environment. The present study was carried out to determine the impact of Malathion an organophosphate pesticide on biochemical constituents i.e. proteins, carbohydrates, and ninhydrine positive substances (Free Amino acids) of fresh water fish Channa punctatus (Bloch). The fish were exposed to the toxicant Malathion at different time intervals i.e. 24, 48, 72 and 96hr and the quantitative variations were observed on biochemical constituents in different tissues of C. punctatus i.e. gill, liver, intestine, muscle and brain. The results revealed that the total proteins, carbohydrates and free aminoacids in different tissues of fish were found to be varied at different time intervals of Malathion exposure and a significant decrease was observed in all the constituents compared to control. The maximum decrease in proteins, carbohydrates and free aminoacids was observed at 96hrs and 72hrs followed by 48hrs and 24hrs in different tissues of fish C. punctatus. Thus our present investigation reports that the period of exposure of Malathion has affected the amount of biochemical constituents in C. punctatus.
Keywords: Organophosphates, Malathion, C. punctatus, proteins, carbohydrates, free aminoaicds.
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Yonar EM, Yonar SM, Sener M and Silici US and Dusukcan M: Protective role of Propolis in Chlorpyrifos induced changes in the haematological parameters and oxidative and anti oxidative status of Cyprinus carpio. Food.Chem.Toxicol. 2012;50:2703-2708.
Apostol. S., The Toxicity of certain pesticides tprotozoans. Proc.Symp.Environ.Biol. 97-102.
Ghosh T.K and Chatterjee S.K.: Influence of Nuvan on the organic reservoirs of Indian fresh water murrel, Channa punctatus. J . Environ. Biol. 1989(10):93- 99
Barbara Texeira E.A. et al. Changes of enzymes activity and protein profiles caused by high-pressure Processing in Sea Bass (Dicentrarchus labrax) Fillets.J.Agric.Food Chem, 2013;61:2851-2860.
Elhalwagya, E.A. et al., Comparative study on Pesticide mixture of Organophosphorous and parathyroid in commercial formulation. Environ.Toxicol.Pharmacolol. 2009;28:219-224.
Selvakumar S, Geraldine P, Shanji S and Jayakumar T: Stress or-specific induction of heat shock protein 70 in the freshwater prawn Macrobrachium malcolmsonii (H. Milne Edwards) exposed to the pesticides endosulfan and carbaryl. Pestic.Biochem. Physiol. 2005;82:125-132.
Tamizhazhagan, V and Pugazhendy K. The toxicity effect of Monocrotophos 36% Ec on the Hematology, Labeo rohita (Hamilton, 1882).Int J Curr Pharm Res. 2015;7(4):92-95.
Tamizhazhagan.V and Pugazhendy.K. The toxicity effect of Monocrotophos 36% E.C on The Biochemical changes Labeo rohita (Hamilton, 1882). International Journal for Scientific Research & Development; 2016;3(11):802-808.
Nagaraju B, Venkatarathnamma V. Effect of Profenophos, an organophosphate on protein levels in some tissues of fresh water fish Labeo rohita (Hamilton).Ijpps, 2013;5(1):274-279.
Kalaimani S and Kandeepan. C. Effect of organophosphate pesticide on the fresh water fish Labeo rohita (Hamilton). Indian Journal of Natural Sciences, 2017, 8(44):12700-12713.
Veeraiah K., Srinivasarao P., Symuktha Rani A., and Dilleswar Rao H: Changes in Biochemical parameters of fresh water fish Labeo rohita exposed to lethal and sub lethal concentration of Indoxacarb. Int.J. of Bioassays. 2013;2(10):1382-1387.
Remia., KM, Logan Kumar S and Rajmohan D. Effect of an Insecticide (Monocrotophos) on some biochemical constituents of fish Tilapia mossambicus. Poll.Res. 2008;27(3):523-526.
Singh RN, Pandey PK, Singh NN, Das VK. Acute toxicity and Biochemical responces of common carp (Cyprinus carpio(Linn.) to an organophosphate (Dimethoate).World Journal of Zoology, 2010;5(3):183-188.
Stalin SI, Das SSM. Biochemical changes in certain tissues of Cirrhinus mrigala (Hamilton) exposed to Fenthion. International Journal Environmental Sciences.2012;2:3.
Justin Raj and Baby Joseph: Impact of Acetamiprid Toxicity on Electrophoretic patterns in Liver, Brain, and Gill tissues of the fish Oreochromis mossambicus. International Journal of Zoological Research. 2017;13:12-124.
Dube S, Shrivatsava R, Chouhan R, Raghuvanshi A, Vinoy K. Shrivatsava. Ameliorative role of Vitamin –C against Dimethoate toxicity in air breathing fish Clarias batrachus) (LINN).Global Journal Bioscience and Biotechnology. 2014;3(1):46-50.
Bheem Rao T, Thirupathi K, Venkaiah Y. Effecct of Methyl Parathion on biochemical contents of fresh water fish Heteropneustes fossilis. 2017;9(7):2869-2874.
Bhuvaneshwari R, Mamatha N, Selvam P, Rajendran RB. Bioaccumulation of metals in muscle, liver and gills of six commercial fish species at Anaikari dam of river Kaveri, South India. International Journal of Applied Biology and Pharmaceutical Technology. 2012;03:08-14.
Kumar S, Singh M: Toxicity of Dimethoate to a fresh water teleost Catla catla. Journal of Experimental Zoology, 2000; 31:83-88.
Tilak K.S, Sattyavardhan K and Tathaji PB: Biochemical changes induced by Fenvelerate in the fresh water fish Channa punctatus . J.Ecotoxicol.Environ.Monit, 2003;13:261-270.
Mathivanan R. Effects of sub lethal concentration of Quinolphos on selected respiratory and Biochemical parameters in the fresh water fish Oreochromis mossambicus. J. Ecotoxicol. Environ. Monit. 2004;14:57-64.
Shrivatsava S and Singh S: Changes in protein content in the muscle of Heteropneustes fossilis exposed to Carbaryl. J.Ecotoxicol.Environ.Monit. 2004;14:57-64.
Padmavathi N, Siva Raju S, Sathic O, Muthukumarvel K. Astudy on the toxicity of pesticide Cypermethrin on the biochemical composition of fresh water fish Mystus vittatus. Int.J. of Zool. And Applied Biosciences. 2017;2(2):95-99.
Finney DJ:Probit Analysis, 3rd edition, London:Cambridge University .Press. 1971;21.
APHA. Standard methods for theexamination of water and wastewater, 18tthEdition, American Public Health Association; 1992.
Lowry OH, Rosenbrogh NJ, Randall RJ. Protein measurements with folin-phenol reagent. J.Biol.Chem. 1951;193:265-267.
Schactele GR, Pollock RL. A Simplified method for the quantitative assay of small amounts of protein in biologic material. Analytical Biochemistry. 1973;51:646-655.
Carrol NV, Longley RW and Roe JH. The determination of glycogen in liver and muscle by use of Anthrone reagent. Journal of Biochemistry 1956; 220:583-593.
Lee YP, Takahashi T. An Improved calorimetric determination of aminoacids with the use of ninhydrin. Analytical Biochemistry, 1966;14:71-77.
Sanjoy Deka and Rita Mahantha. .A study on the effect of Organophosphate pesticide Malathion on Hepato-renal and Reproductive organs of Heteropneustes fossilis (Bloch). The Science Probe. 2012; 1(1):1-13.
Pritchard JB. Environmental Health perspectives Journal Article Aquatic Toxicology Past, Present and Prospects. 1993;100:249-257.
Edwards, C.A. Environmental Pollution by Pesticides. Plenum Press.London. New York; 1973.
Kamble, G.B. and Muley, D.V. 2000. Effect of acute exposure of endosulfan and chlorpyrifos on the biochemical composition of the fresh water fish, Sarotherodon mossambicus. Indian J. Environ. Sci., 4(1): 97-102.
Bhalchandra B. Waykar And V. S. Lomte. Acute Toxicity Of Pesticides Carbaryl And Endosulfan To Fresh Water Bivalve Parreysia Cylindrica. Poll Res.2001, 20 (1) : 25-29
Sindhe, S.C.S., Indira Pala and Butchiram, M.S. 2007. Toxicity and behavioural changes in the fresh water fish, Labeo rohita exposed to Zirum. J. Ecotoxicol. Environ. Monit., 17(6): 537-542.
Arjmandi, R, Tavakol, M. and Shayeghi, M., Determination of organophosphate insecticide residues in the rice paddies. IJEST. 2010;7(1):175- 182.
Umminger BL. Relation of whole blood sugar concentration in vertebrate to standard metabolic rate. Comparative Biochemistry and Physiology. 1997;55:457-460.
Bose S, Nath S, Sahana SS. Toxic Impact of Thiamethoxan on the growth performance and liver protein concentration of a fresh water fish Oreochromis niloticus (Trewavas). Indian .J.Fundam.App.Life Sci. 2011;1:274-280.
Singh RK, Sharma B. Carbofuran induced biochemical changes in Clarias batrachus. Pest. Sci. 1998;53: 285-290.
Gul-e-Zehra Naqvi, Nafisa Shoaib, Aisha Majid Ali. Pesticides impact on protein in fish (Oreochromis mossambicus) tissues. Pesticides impact on protein in fish (Oreochromis mossambicus) tissues. IJGMS. 2017;46(09):1864-1868.
Nagaraju Bantu, Zenebe Hagos, Krishna Chaitanya, Gopala Krishnan, Rathnamma, Abaynew, Zenebe Teka, Mulgeta. Effect of an Insecticide Chlorantraniliprole on Biochemical Characteristics of Snakehead Fish, Channa punctatus (Bloch, 1793). Innovare Journal of Agri. Sci. 2018;6(1):1-7.
Tilak K.S. and Yakobu,. Toxicity and effect of Fenvelerate on fish Ctenopharygodon idellus .J.Ecotoxicol.Environ.Monit. 2002, 12:9-15.
Sathick, O, Farvin Banu, S., Muthukumarvel, K: Impact of pesticide Monocrotophos on selected Biochemical parameters and Histology of liver of Mystus gulino (Hamiltion): RJLPBCS, 2019 5(3):76-83.
Venkataramana Sandhya GV, Rani PN and Moorthy PS: Impact of Malathion on biochemical parameters of gobiid fish Glossogobius giuris (Ham). J. Environ. Biol. 2006;27(1):119-122.
Pathak L, Singh DP and Kumar S: Effect of Malathion on haematological parameters of a fresh water fish, Labeo rohita. Bionotes. 2009;11:68.
Malla Reddy P and Bashamohindeen MD; Toxic Impact of Fenvelerate on protein metabolism in the branchial tissue of fish Cyprinus carpio.Curr.Sci.1988;57:211-212.
Rao JV, Shobha Rani CH, Kavitha P, Nageswararao R, Madhavendra SS. Toxic effects of Chlorpyrifos on Oreochromis moss bicus .Bull.Environ.Contam.Toxicol. 2003;70:985-992.
Jayaprakash G, Raju N, Bheem Rao T, Venkaiah Y. International Journal of Biology, Pharmacy and Allied Sciences (IJBPAS). 2015;4(2):668-677.
Anitha Susan, Shoba K, Veeraiah K, Tilak KS. Studies on Biochemical changes in the tissues of Labeo rohita and Cirrhinus mrigala exposed to Fenvelerate technical grade . J. Toxicol. Envt. Health Sci. 2010; 2(5):53-62.