BUTACHLOR INDUCED SERUM BIOCHEMICAL ANOMALIES CORRELATED WITH HISTOPATHOLOGICAL CHANGES IN INTESTINAL TISSUE OF Clarias batrachus (LINN.): In-vivo STUDY

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GYANENDRA BAHADUR CHAND
https://orcid.org/0000-0002-9356-9719
PRAKRITI VERMA
PRAKASH SINGH
SUDAY PRASAD

Abstract

The present study aimed to investigate the adverse effects of butachlor (2-chloro-2', 6’-diethyl-N - butoxymethyl) acetanilide on serum total protein and glucose level and histo-pathological changes in small intestine especially proximal part (duodenum) of walking catfish Clarias batrachus (Linn.). 96 hour LC50 of butachlor for the fish was determined by the probit regression and then the experimental fishes were exposed to sub lethal dose (1.5µl/ L i.e. 1/36th of LC50) for 7, 14 and 21 days respectively. Blood samples were collected in heparinised syringe by puncturing the caudal vein and the serum was extracted as per standard protocol. The proximal part of intestine was dissected out and fixed in aqueous Bouin’s fixative. Paraffin spread sections were double stained in haemotoxylin and eosine, mounted in DPX and viewed under Olympus 2000 trinocular compound microscope. Light microphotographs were taken in canon ISUS 130X digital camera. Serum total protein and glucose estimation was done as per standard protocol. The LM study of intestinal tissues of treated fish showed degeneration in mucosa, sub-mucosa and muscularis mucosa region of duodenum in comparison to that of control fish.  After 7 and 14 days exposure of butachlor the degeneration were prominent in tunica mucosa and sub mucosa layer as marked by erosion of brush borders of villi and basal lamina, hypertrophy of goblet cells, appearance of large number of vacuoles in sub mucosa region and migration of lymphoid tissues in the lamina proporia. Exposure of butachlor for longer duration of 28 days resulted in widening of luminal region, necrosis of goblet cells, roblet cells as well as entero-endocrine cells. Besides, extensive haemorrhages and congested absorptive region were prominently marked. Biochemical results of treated fish showed significant decrease in serum total protein and abnormal fluctuation in glucose level in contrast to control. A significant (p<0.05) consistent decline in serum protein can be correlated with the diminished absorption rate of protein by the altered intestinal enterocytes under butachlor stress. Hence elevated serum protein level correlated with histopathological alteration in the proximal intestine of fish may be considered as bio-indicator of polluted aquatic bodies.

Keywords:
Butachlor, Clarias batrachus, glucose level, histopathology, small intestine, total protein

Article Details

How to Cite
CHAND, G. B., VERMA, P., SINGH, P., & PRASAD, S. (2021). BUTACHLOR INDUCED SERUM BIOCHEMICAL ANOMALIES CORRELATED WITH HISTOPATHOLOGICAL CHANGES IN INTESTINAL TISSUE OF Clarias batrachus (LINN.): In-vivo STUDY. UTTAR PRADESH JOURNAL OF ZOOLOGY, 42(8), 18-28. Retrieved from https://www.mbimph.com/index.php/UPJOZ/article/view/2067
Section
Original Research Article

References

Khojasteh SM, Sheikhzadeh F, Mohammadnejad D, Azami A. Histological, Histochemical and Ultrastructural study of the intestine of Rainbow Trout (Oncorhynchus mykiss). World Applied Sciences Journal. 2009a;6(11):1525-1531.

FAO. Fisheries and aquaculture department. The State of World Fisheries and Aquaculture. 2010;3-15.

Poet TS, Wu H, Kousba A, Timchalk C. In-vitro hepatic and intestinal metabolism of organophosphate pesticide chloropyriphos and dizinon. Toxicol. Sci. 2003;72 (2):193-200.

Verma P, Chand GB. Altered biochemical profile of carbohydrate, protein and triglycerides in Clarias batrachus and Heteropneustes fossilis due to rogor and Endosulfan toxicity. J. Ecophysiol Occup. Hlth. The Academy of Environmental Biology, India. 2007;7:63-68.

Rady IM. Effects of exposure to Diazinon on the lung and small Intestine of guiea pig, Histological and some Histological Changes. 2009;52(2): 317-326.

Anarse S, Borde S, Humbe A. Histopathological study of Trygon zugei infected with tapeworm from Ratnagiri district (M.S.) India. International Multidisciplinary Research Journal. 2012;2(4):20-21.

Verma P, Chand GB. Butachlor induced oxidative imbalance correlated with gonadal toxicity in fish: In vivo enzymatic and ultastructural study. World Journal of Pharmaceutical Research. 2015;4(8):2327-2340..

EPA. Quantitative usage analysis foe diazinon. Washington, U.S.A. DC; 1999.

Ortiz JB, Gonzalez ML, Sarasquete C. Histological alterations in different tissues of fishes under the impact of persistent chemical pollution. Ecotoxicology Environmental Restontion. 2002;54:45-52.

Reigart JR, Roberts JR. Recognition and management of pesticide poisoning. Fifth ed. Washington DC. U.S. EPA. 1999;34-38.

Nwani CD, Lakra WS, Nagpure NS, Kumar R, Kushwaha B, Srivastava SK. Toxicity of the herbicide atrazine: Effects on lipid peroxidation and activities of antioxidant enzymes in the freshwater fish Channa punctatus (Bloch). International Journal of Environmental Research Public and Health. 20107;3298- 3312.

Merck’s Index. An Encyclopaedia of Chemicals and Natural Products, 11th Edition; 1989.
ISBN: 0-911910-28-X, 1989.

Chang J, Liu S, Zhou S, Wang M, Zhu G. Effect of butachlor on reproduction and hormone level in adult zebra fish (Danio rerio). Exp. Toxicol. Pathol; 2011.
DOI: 10.1016/j.etp.201108.007

Peebua P, Kosiyachinda P, Pockethitiyook K. Evaluation of alacholor herbicide impacts on Nile tilapia (Orechromis niloticus) using biochemical biomarkers. Bulletin of Environ. Conta. And Toxicol. 2007;78(2):138-141.

Geng BR, Yao D, Xue QQ. Acute toxicity of the pesticide dichlorvos and herbicides butachlor to the tadpoles of four anuran species. Bull. Environ. Conta. Toxicol. 2005; 75:343-349.

Tilak KS, Veeraih K, Bhaskara PT, Butchirain MS. Toxicity studies of butachlor to fresh water fish Channa punctatus (Bloch.). J. Envirton. Biol. 2007;28:485-487.

Geng G, Nair U, Yorimitsu KY. Golgi secretory proteins are required for autophagy in Saccharomyces cereviceae. Mol. Biol. Cell. 2010;21(13):2257-69.

Ateeq B, Farah M, Ali M, Ahmad W. Introduction of micronuclei and erytrocytes alter45ation in cat fish Clarias batrachus by 2, 4 dichloro phenoxyacetic acid and butachlor. Mutation Research. 2002;518:135-144.

APHA. Standard method for the examination of water and wastewater (21st Ed). A joint publication of the American Public Health Association (APHA), the American Water Works Association (AWWA), and the Water Environment Federation (WEF). 2005;1368.

Weichselbaum TE, Amer J. J. Clinical Pathology. 16:40.

Henry RJ, Cannon DC, Winkelman JW. Clinical chemistry: Principles and Techniques, Harper and Row, 2nd Edition; 1974.

Kaplan LA. Clinical Chemistry, The C. V. Mosby Co. St. Louis, Baltimore, Philadelphia, Toronto.1989;854-856.

Amer FI, Naguib SAA, Ghafar FA. Comparative study on the intestine of Schilbe mystus and Labeo niloticus in correlation with their feeding habits. Ezypt J. Aquat. Biol. and fish. 2008;12(4):275–309.

Banan Khojasteh SM, Ebrahimi S, Ramezani M, Haghmia H. The hoistological and histochemical studyon the esophagus and intestine in common carp (Cyprinus carpio). J. Anim. Biol. 2009b;1:18-26.

Kumar S, Tembhre M. Anatomy and physiology of fishes. 1st Edn., Nehru Nagar, Ghaziabad, Vikas Publishing House Pvt. Ltd. 1996;65-70.

Albrecht MP, Ferreira MFN, Caramaschi EP. Anatomical features and histology of the digestive tract of two related neotropical omnivorous fishes (Characiformes; Anostomidae). J. Fish Biol. 2001;58:419-430.

Cinar K, Senol N. Histological and histochemical characterization of the mucosa of the digestive tract in flower fish (Pseudophoxinus antalyae). Anat. Histol. Embryol. 2006;35:147-151.

Banan Kojasteh SM. The morphology of the post gastric alimentary canal in teleost fishes: A brief review. International Journal of Aquatic Sciences. 2012;3(2):71-88.

Stoskof MK. Fish medicines: W B Saunder’s Company. 1993;35-39.

Powell MD, Briand HA, Wright GM, Burka JF. Rainbow trout (Onchorhynchus mykiss Walbaeum) intestinal eosinophilic granule cells (EGC) response to Aeromonas salmonicida and Vibrio anguillarum extracellular products. Fish, Shellfish Immunology. 1993;3:279- 289.

Genten F, Trewinghi E, Danguy A. Digestive system in: Atlas of Fish Histology, Science Publishers. 2009;75-91.

Kapoor BC, Smith H. Verighina IA. The alimentary canal and digestion in teleosts. Adv. Mar. Biol. 1975;13:109-239.

Yamamoto T. An electron microscopic study of the columnar epithelial cell in the intestine of fresh water teleosts: Goldfish (Carassius auratus) and Rainbow trout (Salmo irideus). Zeit. Zekkfirsch.1966;72:66-87.

Frierson EW, Foltz JW. Comparison and estimation of absorptive intestinal surface areas in two species of cichlid fish. Trans. Am. Fish. Soc. 1992;121:517- 523.

Kuzmina VV, Gelman IL. Membrane linked digestion in fish. Rev. Fish. Sci. 1997;5:99- 129.

Reifel CW, Travill AA. Structure and carbohydrate histochemistry of the intestine in ten teleostean species. J. Morph. 1979;162:343-360.

Petrinec Z, Nejedli S, Kuzir S, Opacak A. Muco-substances of the digestive tract mucosa in northern pike (Esox lucius L.) and European catfish (Silurus glanis L.). Veterinarski. Arhiv. 2005;75:317-372.

Bozic F, Srebocan E, Kozaric Z. Starvation-induced patho-biology in the gut of carp (Cyprinus carpio L.). Berl. Munch. Tierarztl. Wschr. 2001;114:134-138.

Teresa O. Developmental changes of digestive system structures in pike-perch (Sander lucio perca L.). Electronic. J. Ichthyol. 2005;2:65-78.

Reite OB. Mast cells/eosinophilic granule cells of teleostean fish: A review focusing on staining properties and functional responses. Fish. Shellfish. Immun. 1998;8:489-513.

Holmgren S, Olsson C. The neuronal and endocrine regulation of gut function. In: Fish Neuroendocrinology. Academic Press. 2009;467-512.

Murray HM, Wright GM, Goff GP. A comparative histology and histochemical study of the stomach from three species of pleuronectid, the Atlantic halibut, Hippoglossus hippoglossus, the yellow tail flounder, Pleuronectes ferruginea, and the winter flounder, Pleuronectes americanus. Can. J. Zoology. 1994;72:1199-1210.

Murray HM, Wright GM, Goff, GP. A comparative histological and histochemical study of the post-gastric alimentary canal from three species of pleuronectids, the Atlantic halibut, the Yellow tail flounder and the Winter flounder. J. Fish. Biol. 1996;48:187- 206.

Raji AR, Norouzi E. Histological and Histochemical study on the alimentary canal in Walking catfish (Claris batrachus) and Piranha (Serrasalmus nattereri). Iranian Journal of Veterinary Research, Shiraj University. 2010;11(3):32.

Ritter EJ. Altered biosynthesis. In: Handbook of teratology. Plenum Press, New York. 1977;2.

Verma P, Mishra BB, Rani P. Influence of Endocel and Rogor on serum free amino acid and total protein level in Clarias batrachus (Linn.) Journal of Environmental Biology. Triveni Enterprises. 2015;36(3):639-643.