LIPOLYTIC ACTIVITY IN LARVAL HAEMOLYMPH OF HELICOVERPA ARMIGERA (HUBNER)
AISHWARYA S. PAWAR *
DEPARTMENT OF ZOOLOGY, SHRI.VIJAYSINHA YADAV ARTS AND SCIENCE COLLEGE, PETH VADGAON- 416 112, INDIA.
R.M. GEJAGE *
DEPARTMENT OF ZOOLOGY, SMT. KUSUMTAI RAJARAMBAPU PATIL, KANYA MAHAVIDYALAYA, ISLAMPUR- 415 409, INDIA
R.S. DUBAL *
PG-DEPARTMENT OF ZOOLOGY, YASHWANTRAO CHAVAN INSTITUTE OF SCIENCE, SATARA- 415 001, INDIA
*Author to whom correspondence should be addressed.
Abstract
Lipolytic activity in larval haemolymph of gram pod borer, Helicoverpa armigera (Hubner) revealed optimum pH 7.8, incubation time 20 minutes, temperature 37° C, enzyme concentration 1% and substrate concentration 6% and Michaelis-Menten constant 0.12 x 10-2 mM. The gradual increase in larval haemolymph lipase activity was observed from 7-day larvae to 10-day larvae and decrease from 10-day larvae to 15-day larvae. The maximum lipase activity was observed in 10-day larvae. The physiological role of larval haemolymph lipase (EC 3.1.1.3) of H. armigera has been reported in present paper.
Keywords: Lipase, haemolymph, larva, insect, H. armigera