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The objective of current research is to prove the presence of bacteria genus Bacillus in hot mineral water from Rupite, Bulgaria. The water of Rupite is rich in hydrocarbon and calcium ions. The presence of bivalent Calcium (Ca2+) and Magnesium (Mg2+) ions in warm and hot mineral waters take part in the activation of cortex-lytic enzymes during germination of Bacillus subtilis (Igura, 2003).
In 2010 Ignatov and Mosin indicate the possibility for origin of life and living matter in hot mineral water 3.5 billion years ago. Comparative analyses are conducted based on experiments for the similarities of spectra of hot mineral waters and cactus juice. The closest matches are the spectra of cactus juice and the water from Rupite, Bulgaria. Such compatibility is not observed between the cactus juice and sea water. In the cactus juice and the hot mineral water we observed five comparable local extremums (8.95; 9.67; 9.81; 10.47; 11.13 μm), whereas there was only one (9.10 μm) with the sea water. It is therefore justified to suggest that most probably life originated in hot mineral water. According to their temperature they can be cold (up to 37 °С), warm (from 37°C to 60 °C) and hot (over 60 °C). This is temperature standard in Bulgaria, European Union.
Han. Extracellular and intracellular biomineralization induced by Bacillus licheniformis DB1-9 at Different Mg/Ca Molar Ratios; Minerals. 2018;8(12):585.
Igura N et al. Effects of minerals on resistance of Bacillus subtilis spores to heat and hydrostatic pressure. Applied and Environmental Microbiology. 2003;69(10):6307-10.
Mudgil et al. Biomineralization potential of Bacillus subtilis, Rummeliibacillus Stabekisii and Staphylococcus Epidermidis Strains In Vitro Isolated from Speleothems, Khasi Hill Caves, Meghalaya, India. Geomicrobiology Journal. 2018;35(8).
Spadafora A et al. Microbial biomineralization processes forming modern Ca:Mg carbonate stromatolites, Sedimentology; 2009.
Barabesi Ch et al. Bacillus subtilis Gene cluster involved in calcium carbonate biomineralization, Journal of Microbiology. 2007;189(1):228-35.
Ignatov I. Which water is optimal for the origin (generation) of life? Euromedica, Hanover. 2010;34-37.
Ignatov I. Entropy and time in living organisms, ArchivEuromedica, Hanover, 1st&2nd Edition. 2011;74-75.
Yanchulina FS. Quantum nonlocal bonds between living organisms and role in evolution. Canadian Journal of Pure and Applied Sciences. 2018;12(3): 4651-4658.
Ignatov I, Mosin OV. Possible processes for origin of life and living matter with modeling of physiological processes of bacterium Bacillus Subtilis in heavy water as model system. Journal of Natural Sciences Research. 2013;3(9):65-76.
Ignatov I, Mosin OV. Deuterium, heavy water and origin of life. LAP LAMBERT Academic Publishing. 2016;1-500.
Koonin EV. The Cosmological model of eternal inflation and the transition from chance to biological evolution in the history of life. Biology Direct. 2007;2:15.
Damer B, Deamer D. Coupled phases and combinatorial selection of fluctuating hydrothermal pools: A scenario to guide experimental approaches to the origin of cellular life. Life. 2015;5(1):872–887.
Kambourova M. et al. Anoxybacillus rupiences sp. Nov. a novel thermophylic bacterium isolated from Rupi basin (Bulgaria), Extremophiles. 2007;11:577-583.
Kambourova M. et al. Archaeal and bacterial diversity in two hot springs from geothermal regions in Bulgaria as demostrated by 16S rRNA and GH-57 genes, International Microbiology. 2015;18:217-223.
Strunesky O, Pilarski P et al. High diversity of thermophilic cyanobacteria in rupite hot spring identified by microscopy, Cultivation, Single-cell PCR and Amplicon sequencing, Extremophiles. 2009;23(1):35-48.
Mosin OV, Shvets VI, Skladnev DA, Ignatov I. Microbiological synthesis of [2H]-Inosine with a high degree of isotopic enrichment by the gram-positive chemoheterotrophic bacterium Bacillus subtilis. Applied Biochemistry and Microbiology. 2013; 49(3):233-243.
Zavarzin GA. Microbial geochemical Calcium cycle, Microbiology, 2002; 71;1-17.
Waterworth S, et al. Metabolic specializations within a bacterial community to create living rocks, Biorxiv; 2019.
Bosak T, Greene SE, Newman DK. A likely role for anoxygenic photosynthetic microbes in the formation of ancient stromatolites. Geobiology. 2007;5:119–126.
Ignatov I. Origin of life in hot mineral water from hydrothermal springs and ponds. Effects of hydrogen and nascent hydrogen. Analyses with spectral Methods, pH and ORP, European Reviews of Chemical Research. 2019;6(2):49-60.
Vassileva P, Voykova D, Ignatov I, Karadzhov S, Gluhchev S, Ivanov N, Mehandjiev D. Results from the research of water catholyte with nascent (Atomic) Hydrogen. Journal of Medicine, Physiology and Biophysics. 2019; 52:7-11.
Mörtelmaier Ch. et al. Identification performance of MALDI-ToF-MS upon mono- and bi-microbial cultures is cell number and culture proportion dependent. Analytical and Bioanalytical Chemistry. 2009;411:7027-7038.
Protocol for research is from Regional Health Inspectorate, Haskovo, Bulgaria, 1.03.2020 –Rupite 1, Decree № 14 regarding the resort resources, resort localities and resorts (Official State Gazette, issue 79 from 1987, and amendment published in issue 70 from 2004).
Verma J et al. Characterization and screening of thermophilic Bacillus strains for developing plant growth promoting consortium from hot Spring of leh and Ladakh region of India. Frontiers in Microbiology; 2018.
Aanniz T et al. Thermophilic bacteria in Moroccan hot springs, Salt marshes and desert soils, Brazilian Journal of Microbiology. 2015;46(2):443-453.
Hair OJ. et al. Draft genome sequence of Bacillus altitudinis YNP4-TSU, isolated from Yellowstone National Park. American Society for Microbiology. 2017;5(28):e00631-17.
Colón Santos S-M. Exploring the untargeted synthesis of prebiotically-plausible molecules. PhD thesis, University of Glasgow.
Ignatov I, Mosin OV. Water and Origin of Life: Collection of Scientific Publications, Moscow, Berlin, Direct Media. 2016;1-658.
Turner R. Essential in Microbiology, Ed-Tech Press. 2018;1-289.
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